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JoVE Journal
Genetics
Author Produced

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Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9
 
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Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9

Article doi: 10.3791/58130
August 25th, 2018

Summary August 25th, 2018

Please note that all translations are automatically generated.

Click here for the English version.

Described here is a protocol for tagging endogenously expressed proteins with fluorescent tags in human induced pluripotent stem cells using CRISPR/Cas9. Putatively edited cells are enriched by fluorescence activated cell sorting and clonal cell lines are generated.

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