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Journal / Genetics / Endogene Protein merking i menneskelig indusert…
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Genetics
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JoVE Journal Genetics
Endogene Protein merking i menneskelig indusert Pluripotent stilk celler med CRISPR/Cas9
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Endogene Protein merking i menneskelig indusert Pluripotent stilk celler med CRISPR/Cas9

Article DOI: 10.3791/58130-v • 14:49 min • August 25th, 2018
August 25th, 2018 •
1, 1, 1, 1, 1, 1
1Allen Institute for Cell Science

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Beskrevet her er en protokoll for merking endogenously uttrykt proteiner med fluorescerende koder i menneskelig indusert pluripotent stamceller ved hjelp CRISPR/Cas9. Åpenbart redigerte celler er beriket med fluorescens aktivert celle sortering og klonal cellelinjer genereres.

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Genetikk problemet 138 stilk celler hiPSC molekylær biologi CRISPR Cas9 genomet Engineering Gene Knock-in Gene Tagging fluorescerende Protein
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