Use of Hematopoietic Stem Cell Transplantation to Assess the Origin of Myelodysplastic Syndrome

This method can help answer a few questions in the method of malignancy field about characterization of a cell that initiate a malignancy. The main advantage of this technique is that it provides a foundation for the functional analysis of these cancer stem cells. The implications of this technique extend to where the therapy of myelodysplastic syndromes or MDS, as it can aid in the identification of the disease initiating cells.

To prepare the recipients for adoptive transfer, one week before the transplant provide sterile water supplemented with 100 milligrams per liter of ciprofloxacin to con genic recipient ANIMALS for seven days in a specific, pathogen free animal facility. On the seventh day, have a trained, certified cesium 17 operator set the cesium source instrument to deliver 70 centigrayS of total body gamma irradiation per minute. Place 10 mice in a custom-designed holder.