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JoVE Journal Immunology and Infection
Avaliação da Interface sináptica de células T humano primário do sangue periférico e tecido linfoide
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Avaliação da Interface sináptica de células T humano primário do sangue periférico e tecido linfoide

Article DOI: 10.3791/58143-v • 06:27 min • July 30th, 2018
July 30th, 2018 •
1, 1, 2,3, 2, 4
1Department of Microbiology and Immunology, Thomas Jefferson University, 2Department of Microbiology and Institute for Immunology, Perelman School of Medicine, University of Pennsylvania, 3Department of Medicine Huddinge, Karolinska Institutet, Karolinska University Hospital Huddinge, 4Departments of Microbiology and Immunology and Medical Oncology, Sidney Kimmel Cancer Center, Thomas Jefferson University

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O protocolo descreve uma técnica para estudar a capacidade das células T policlonais humanas de primário para formar sinápticas interfaces usando bilayers do lipid planar. Usamos esta técnica para mostrar a capacidade de formação de sinapse diferencial de células T humanas primárias derivado dos gânglios linfáticos e sangue periférico.

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Imunologia e infecção edição 137 células T humanas primárias sangue periférico gânglios linfáticos bilayers do lipid planar estrutura interface sináptica
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