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Immunology and Infection

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JoVE Journal Immunology and Infection
Beoordeling van de synaptische Interface van primaire menselijke T-cellen uit perifeer bloed en lymfoïde weefsel
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Beoordeling van de synaptische Interface van primaire menselijke T-cellen uit perifeer bloed en lymfoïde weefsel

Article DOI: 10.3791/58143-v • 06:27 min • July 30th, 2018
July 30th, 2018 •
1, 1, 2,3, 2, 4
1Department of Microbiology and Immunology, Thomas Jefferson University, 2Department of Microbiology and Institute for Immunology, Perelman School of Medicine, University of Pennsylvania, 3Department of Medicine Huddinge, Karolinska Institutet, Karolinska University Hospital Huddinge, 4Departments of Microbiology and Immunology and Medical Oncology, Sidney Kimmel Cancer Center, Thomas Jefferson University

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Het protocol beschrijft een techniek om te bestuderen van de mogelijkheid van primaire polyklonale menselijke T cellen vormen van synaptic interfaces met behulp van vlakke lipide bilayers. We gebruiken deze techniek te laten zien van de differentiële synapse formatie vermogen van menselijke primaire T-cellen afgeleid van lymfeklieren en perifeer bloed.

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Immunologie en infecties probleem 137 primaire menselijke T cellen perifeer bloed lymfeklieren vlakke lipide bilayers structuur synaptic interface
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