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Cancer Research

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JoVE Journal Cancer Research
Live-Cell Imaging Assays to Study Glioblastoma Brain Tumor Stem Cell Migration and Invasion
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Live-Cell Imaging Assays to Study Glioblastoma Brain Tumor Stem Cell Migration and Invasion

Article DOI: 10.3791/58152-v • 09:36 min • August 29th, 2018
August 29th, 2018 •
1, 1, 1,2, 1, 1
1Hotchkiss Brain Institute, Department of Cell Biology and Anatomy, Cumming School of Medicine, University of Calgary, 2Department of Developmental and Stem Cell Biology Program, Arthur and Sonia Labatt Brain Tumor Research Centre, The Hospital for Sick Children

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Summary
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Here, we describe live-cell imaging techniques to quantitatively measure the migration and invasion of glioblastoma brain tumor stem cells over time and under multiple treatment conditions.

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Live-cell Imaging Assays Glioblastoma Brain Tumor Stem Cell Migration Invasion BTSC Biology Quantification Multiple Conditions Glioblastoma Therapy Cancer Cells Normal Brain Tissue Disease Recurrence Cancer Stem Cells Cryogenically-preserved BTSCs Thawing Media Centrifugation Resuspend Culture Flask Non-adherent Conditions Neurospheres
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