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Journal / Bioengineering / Microscopia a luce correlativa (CLEM) per il…
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Bioengineering

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JoVE Journal Bioengineering
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Microscopia a luce correlativa (CLEM) per il monitoraggio e la proteina virale di Imaging strutture in cellule di Cryo-immobilizzata associate

Article DOI: 10.3791/58154-v • 09:18 min • September 7th, 2018
September 7th, 2018 •
1, 2, 1, 3, 1, 1, 2,4, 2
1European Molecular Biology Laboratory, 2Department of Infectious Diseases, Molecular Virology, Heidelberg University, 3Central Facility for Electron Microscopy, Ulm University, 4Heidelberg Partner Site, German Center for Infection Research

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Un metodo di microscopia luce correlativa (CLEM) viene applicato alle strutture intracellulari indotte da virus immagine tramite microscopia elettronica (EM) in cellule che sono già stati scelti da microscopia chiara (LM). LM ed EM sono combinati in un approccio di imaging ibrido per ottenere una visione integrata delle interazioni virus-ospite.

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Ad alta pressione di bioingegneria problema 139 congelamento congelare sostituzione vetrificazione rifilatura mirato microscopia elettronica a trasmissione dischi zaffiro coltura cellulare virus infezione interazioni virus-ospite ultrastruttura indotta da virus alterazioni cellulari.
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