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Journal / Biochemistry / 利用商用仪器和自由开源亮度分析软件对蛋白质的聚齐聚进行无标定的体外定量化…
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Biochemistry

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JoVE Journal Biochemistry
利用商用仪器和自由开源亮度分析软件对蛋白质的聚齐聚进行无标定的体外定量化
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Journal (Biochemistry)
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Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry
Journal (Biochemistry)
Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry
 
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利用商用仪器和自由开源亮度分析软件对蛋白质的聚齐聚进行无标定的体外定量化

Article DOI: 10.3791/58157-v • 08:22 min • July 17th, 2018
July 17th, 2018 •
1, 1, 2, 2, 2, 1,2,3,4
1Cellular Imaging Group, Wellcome Centre Human Genetics, University of Oxford, 2Division of Structural Biology, Wellcome Centre Human Genetics, University of Oxford, 3Dynamic Structural Virology Group, Biocruces Health Research Centre, 4IKERBASQUE, Basque Foundation for Science

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本协议描述了一种无标定的方法, 用于在荧光涨落光谱的基础上, 利用商用光扫描显微镜定量测定蛋白质的体外齐聚。给出了正确的采集设置和分析方法。

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生物化学 问题 137 二聚化 校准自由光谱学 荧光涨落 数字和亮度 蛋白质聚集 单分子显微镜
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