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Forberedelse med en høy kvalitet primært cellekulturer fra fisk Pituitaries
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Forberedelse med en høy kvalitet primært cellekulturer fra fisk Pituitaries

Article DOI: 10.3791/58159-v • 07:36 min • August 28th, 2018
August 28th, 2018 •
1, 1, 1, 1, 2, 1
1Department of Basic Sciences and Aquatic Medicine, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, 2Department of Oral Biology, Faculty of Dentistry, University of Oslo

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Her beskriver vi en protokoll for å forberede og opprettholde primære hypofysen cellekulturer fra medaka (Oryzias latipes). Optimalisert forholdene i denne protokollen ta viktige parametre som temperatur, osmolality og pH hensyn mimicking de fysiologiske forholdene av fisk, og dermed gjør fysiologisk mer meningsfulle resultater.

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Nevrovitenskap problemet 138 hypofysen endokrine celler primære kultur fisk dissosiasjon temperatur osmolality pH pCO2 medaka
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