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Journal / Biochemistry / Immobilisation covalente de protéines pour la…
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Biochemistry

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JoVE Journal Biochemistry
Immobilisation covalente de protéines pour la spectroscopie de Force seule molécule
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Immobilisation covalente de protéines pour la spectroscopie de Force seule molécule

Article DOI: 10.3791/58167-v • 11:13 min • August 20th, 2018
August 20th, 2018 •
1,2,3, 2, 1,3, 3, 2, *4, *1,3
1Center for Applied Tissue Engineering and Regenerative Medicine, Munich University of Applied Sciences, 2FG Protein Biochemistry & Cellular Microbiology, Munich University of Applied Sciences, 3Center for Nano Science, Ludwig-Maximilians-Universität München, 4Klinik für Unfallchirurgie, Orthopädie und Plastische Chirurgie, University Medical Center Göttingen
* These authors contributed equally

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Ce protocole décrit l’immobilisation covalente de protéines avec un hétérobifonctionnel agent de couplage silane aux surfaces d’oxyde de silicium conçu pour la spectroscopie de force force atomique microscopie basée seule molécule qui est illustrée par la interaction de RrgA (adhésine pilus-1 pointe de S. pneumoniae) avec la fibronectine.

Tags

Biochimie numéro 138 immobilisation covalente protéine surface silanisation hétérobifonctionnel PEG réactif chimie EDC/NHS amine primaire atomic force spectroscopie de microscopie AFM molécule unique force SMF
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