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Journal / Biochemistry / Covalente immobilisatie van eiwitten voor de…
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Biochemistry

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JoVE Journal Biochemistry
Covalente immobilisatie van eiwitten voor de enkel molecuul Force spectroscopie
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Covalente immobilisatie van eiwitten voor de enkel molecuul Force spectroscopie

Article DOI: 10.3791/58167-v • 11:13 min • August 20th, 2018
August 20th, 2018 •
1,2,3, 2, 1,3, 3, 2, *4, *1,3
1Center for Applied Tissue Engineering and Regenerative Medicine, Munich University of Applied Sciences, 2FG Protein Biochemistry & Cellular Microbiology, Munich University of Applied Sciences, 3Center for Nano Science, Ludwig-Maximilians-Universität München, 4Klinik für Unfallchirurgie, Orthopädie und Plastische Chirurgie, University Medical Center Göttingen
* These authors contributed equally

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Dit protocol beschrijft de covalente immobilisatie van eiwitten met een heterobifunctional silane koppelmiddel op siliciumoxide oppervlakken ontworpen voor de atomaire kracht microscopie gebaseerd enkel molecuul kracht spectroscopie die wordt geïllustreerd door de interactie van RrgA (pilus-1 tip antistoffen van S. pneumoniae) met fibronectine.

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Biochemie kwestie 138 covalente eiwit immobilisatie oppervlakte silanization heterobifunctional PEG reagens EDC/NHS chemie primaire amine atomic force microscopie AFM enkel molecuul kracht spectroscopie de SBF
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