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Journal / Biochemistry / Kovalent immobilisering av proteiner för den enda …
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
Kovalent immobilisering av proteiner för den enda molekyl kraft spektroskopin
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Kovalent immobilisering av proteiner för den enda molekyl kraft spektroskopin

Article DOI: 10.3791/58167-v • 11:13 min • August 20th, 2018
August 20th, 2018 •
1,2,3, 2, 1,3, 3, 2, *4, *1,3
1Center for Applied Tissue Engineering and Regenerative Medicine, Munich University of Applied Sciences, 2FG Protein Biochemistry & Cellular Microbiology, Munich University of Applied Sciences, 3Center for Nano Science, Ludwig-Maximilians-Universität München, 4Klinik für Unfallchirurgie, Orthopädie und Plastische Chirurgie, University Medical Center Göttingen
* These authors contributed equally

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Det här protokollet beskriver kovalent immobilisering av proteiner med heterobifunctional silan kontaktmedel kiseloxid ytor avsedda för den atomic force microscopy baserat enda molekyl kraft spektroskopin som exemplifieras av den växelverkan av RrgA (pilus-1 tip adhesin av S. pneumoniae) med Fibronektin.

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Fråga 138 surface silanisering kovalent protein immobilisering biokemi heterobifunctional PEG reagens EDC/NHS kemi primära aminen atomic force microscopy AFM enda molekyl kraft spektroskopi SMFS
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