Biochemistry
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蜜蜂匀浆中磷脂酶 C 活性的检测
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Summary September 14th, 2018
Please note that all translations are automatically generated.
Click here for the English version.
为了检验药理学药物对蜜蜂脑不同区域磷脂酶 C (plc) 的抑制作用, 我们提出了一种生化检测方法来测量这些地区的 plc 活性。该方法可用于比较组织中的 PLC 活动, 以及不同行为的蜜蜂之间的对比。
Transcript
这种方法可以帮助回答神经科学领域的关键问题，例如PLC在不同大脑区域的活动是否和如何参与蜜蜂的大脑功能。这项技术的主要优点是，研究人员可以很容易地用标准实验室设施进行实验，因为该协议只需要基本的操作。首先，在冰上放置一个50毫升的管子，里面装着蜜蜂30分钟，对蜜蜂进行麻醉。接下来，准备解剖阶段，将一块牙蜡折叠成两半，放在塑料盘中。在双目显微镜下，用钳子将蜜蜂的头与身体分开。然后，将头部固定在牙蜡上，并在每个天线底部插入昆虫针，使头部内部处于向上位置。接下来，将足够的盐水溶液倒入头部以盖住它。使用钳子卸下天线。在此之后，使用手术刀在天线底座附近进行水平切割，并在复合眼的边界进行纵向切割。在头顶上做一个水平切口，取出角质层，打开大脑的窗户。接下来，使用钳子从骨囊和气管中去除大脑内表面的视网膜眼。然后，将钳子直接插入眼皮下，以去除复合眼皮和视网膜眼之间的结缔组织。之后，去除复合眼的角质层和视网膜眼。接下来，使用钳子小心地从大脑内部表面取出剩余的气管。然后，在大脑之间和组织周围切割结缔组织，从头部胶囊解剖大脑。使用钳子，从大脑后表面剥下气管。然后使用手术刀切断蘑菇体或MB和垂直叶旁边的其他大脑区域之间的连接。将解剖的MB和任何剩余的脑组织放入1.5ml管中，用干冰快速冷冻。首先，在冷冻脑组织中加入10ml均质缓冲液。使用塑料 peselle 手动使 1.5ml 管中的组织均质化。在此之后，将均质组织溶液转移到地段的下一个组织，并重复均质化过程。然后，添加 30
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