Chapters
Summary
Herpesvirus of turkeys (HVT) is widely used as a vector platform for the generation of recombinant vaccines against a number of avian diseases. This article describes a simple and rapid approach for the generation of recombinant HVT-vectored vaccines using an integrated NHEJ-CRISPR/Cas9 and Cre-Lox system.
Tags
Recombinant Avian Herpesvirus Vectors CRISPR/Cas9 Gene Editing Viral Antigens Recombinant Vaccines GFP Expression Cassette Cre-LoxP System Multivalent Recombinant Vaccines Chick Embryo Fibroblasts Cas9 Guide RNA Plasmids Donor Plasmid Transfection Reagent HVT Virus Stock Plaque Forming Units
PLAYLIST
CRISPR- genomic scissors of the modern era
Tweaking the genome using the CRISPR/Cas9 suite
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Research • BiologyGeneration of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9 -
Research • GeneticsCRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans -
Research • GeneticsCell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens -
Research • BioengineeringCRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art -
Research • GeneticsCRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation
CRISPR in Cancer
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Research • Cancer ResearchUsing CRISPR/Cas9 to Knock Out GM-CSF in CAR-T Cells -
Research • Cancer ResearchVirus Delivery of CRISPR Guides to the Murine Prostate for Gene Alteration -
Research • Cancer ResearchInvestigation of Genetic Dependencies Using CRISPR-Cas9-based Competition Assays -
Research • Cancer ResearchUsing CRISPR/Cas9 Gene Editing to Investigate the Oncogenic Activity of Mutant Calreticulin in Cytokine Dependent Hematopoietic Cells -
Research • GeneticsInvestigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells -
Research • GeneticsHighly Efficient Gene Disruption of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9 -
Research • Cancer ResearchProstate Organoid Cultures as Tools to Translate Genotypes and Mutational Profiles to Pharmacological Responses
CRISPR in HIV
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Research • Immunology and InfectionCRISPR-Cas9-based Genome Engineering to Generate Jurkat Reporter Models for HIV-1 Infection with Selected Proviral Integration Sites -
Research • GeneticsA Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells -
Research • GeneticsAmplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing -
Research • Immunology and InfectionEvaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy -
Research • GeneticsUsing a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format
CRISPR in tweaking the disease genome
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Research • GeneticsIntroducing a Gene Knockout Directly Into the Amastigote Stage of Trypanosoma cruzi Using the CRISPR/Cas9 System -
Research • GeneticsOverexpressing Long Noncoding RNAs Using Gene-activating CRISPR -
Research • GeneticsEmbryo Microinjection Techniques for Efficient Site-Specific Mutagenesis in Culex quinquefasciatus -
Research • Immunology and InfectionLentiviral CRISPR/Cas9-Mediated Genome Editing for the Study of Hematopoietic Cells in Disease Models -
Research • Immunology and InfectionGenerating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing
CRISPR in helping IVF and emryo transfer technology
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Research • GeneticsUse of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice -
Research • BioengineeringProduction of Genetically Engineered Golden Syrian Hamsters by Pronuclear Injection of the CRISPR/Cas9 Complex -
Research • BiologySilencing the Spark: CRISPR/Cas9 Genome Editing in Weakly Electric Fish -
Research • GeneticsCRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy
CRISPR in improving crop yield and plant biotechnology
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Research • BiologyAgrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice -
Research • BiologyHigh-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots -
Research • GeneticsTransforming, Genome Editing and Phenotyping the Nitrogen-fixing Tropical Cannabaceae Tree Parasponia andersonii -
Research • GeneticsA Robotic Platform for High-throughput Protoplast Isolation and Transformation -
Research • BioengineeringMicroinjection of Western Corn Rootworm, Diabrotica virgifera virgifera, Embryos for Germline Transformation, or CRISPR/Cas9 Genome Editing
CRISPR in stem cell applications/transgenic models
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Research • GeneticsEndogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9 -
Research • Developmental BiologyEstablishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation -
Research • Developmental BiologyGenerating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells -
Research • Developmental BiologyCRISPR/Cas9 Technology in Restoring Dystrophin Expression in iPSC-Derived Muscle Progenitors -
Research • GeneticsIntroducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing
