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Journal / Immunology and Infection / Påvisning af lav kopi nummer integreret Viral DNA …
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Immunology and Infection

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JoVE Journal Immunology and Infection
Påvisning af lav kopi nummer integreret Viral DNA dannet af In Vitro Hepatitis B infektion
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Påvisning af lav kopi nummer integreret Viral DNA dannet af In Vitro Hepatitis B infektion

Article DOI: 10.3791/58202-v • 11:14 min • November 7th, 2018
November 7th, 2018 •
1,2, 1,3
1Department of Infectious Diseases, Molecular Virology, Heidelberg University Hospital, 2Gastroenterology and Liver Research Laboratory, Ingham Institute, 3German Center for Infection Research (DZIF)

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Vi beskriver her in vitro- generation af HBV DNA via en Hepatitis B virus infektion system og meget følsomme påvisning af dets (1 – 2 kopier) integration ved hjælp af inverse indlejret PCR.

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Immunologi og infektion sag 141 HBV DNA integration Myrcludex B hepatocellulært carcinom dobbelt-strenget lineære DNA inverse indlejrede PCR virus løsning viral vedholdenhed
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