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Journal / Biochemistry / Ein Oligonukleotid-basierte Tandem RNA Isolierung …
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Biochemistry

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JoVE Journal Biochemistry
Ein Oligonukleotid-basierte Tandem RNA Isolierung Vorgehensweise eukaryotischen mRNA-Protein-komplexe
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Ein Oligonukleotid-basierte Tandem RNA Isolierung Vorgehensweise eukaryotischen mRNA-Protein-komplexe

Article DOI: 10.3791/58223-v • 09:45 min • August 18th, 2018
August 18th, 2018 •
*1, *1, 1
1Dept. of Microbial Sciences, School of Biosciences and Medicine, Faculty of Health and Medical Sciences, University of Surrey
* These authors contributed equally

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Ein Tandem RNA Isolierung (Reise) für die Verwertung von endogen gebildeten mRNA-Protein-komplexe Vorgehensweise wird. Speziell, RNA-Protein-komplexe sind vernetzt in VivoPolyadenylated RNAs sind isoliert aus Extrakten mit oligo(dT) Perlen und besonderen mRNAs werden mit modifizierten RNA antisense Oligonukleotide erfasst. Proteine gebunden, mRNAs werden vom Immunoblot Analyse erkannt.

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Frage 138 RNA-bindende Protein posttranskriptionelle Genregulation Biochemie Polyadenylated RNA Ribonucleoprotein Komplex antisense-Oligonukleotid Hefe Caenorhabditis Elegans menschliche Zellen
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