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Journal / Biochemistry / En oligonukleotid-baserade Tandem RNA isolering…
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Biochemistry

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JoVE Journal Biochemistry
En oligonukleotid-baserade Tandem RNA isolering förfarande att återställa eukaryota mRNA-proteinkomplex
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En oligonukleotid-baserade Tandem RNA isolering förfarande att återställa eukaryota mRNA-proteinkomplex

Article DOI: 10.3791/58223-v • 09:45 min • August 18th, 2018
August 18th, 2018 •
*1, *1, 1
1Dept. of Microbial Sciences, School of Biosciences and Medicine, Faculty of Health and Medical Sciences, University of Surrey
* These authors contributed equally

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En tandem RNA isolering (resa) för återvinning av endogent bildade mRNA-proteinkomplex beskrivs. Specifikt, RNA-proteinkomplex är tvärbundna i vivo, polyadenylated RNAs isoleras från extrakt med oligo(dT) pärlor och särskilt mRNA fångas med modifierade RNA antisense oligonukleotider. Proteiner som bunden till mRNA upptäcks av immunoblot analys.

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Fråga 138 post-transcriptional genreglering RNA-bindande protein biokemi polyadenylated RNA ribonukleoprotein komplexa antisense oligonukleotiden jäst Caenorhabditis elegans mänskliga celler
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