Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Visualizing Axonal Growth Cone Collapse and Early …
JoVE Journal
Neuroscience

A subscription to JoVE is required to view this content.

JoVE Journal Neuroscience
Visualizing Axonal Growth Cone Collapse and Early Amyloid β Effects in Cultured Mouse Neurons
An Isolated Retinal Preparation to Record Light Response from Genetically Labeled Retinal Ganglion Cells
Journal (Neuroscience)
An Isolated Retinal Preparation to Record Light Response from Genetically Labeled Retinal Ganglion Cells
Electrophysiological Method for Recording Intracellular Voltage Responses of Drosophila Photoreceptors and Interneurons to Light Stimuli In Vivo
Journal (Neuroscience)
Electrophysiological Method for Recording Intracellular Voltage Responses of Drosophila Photoreceptors and Interneurons to Light Stimuli In Vivo
 

Visualizing Axonal Growth Cone Collapse and Early Amyloid β Effects in Cultured Mouse Neurons

Article DOI: 10.3791/58229-v • 06:23 min • October 30th, 2018
October 30th, 2018 •
1
1Division of Neuromedical Science, Institute of Natural Medicine, University of Toyama

Chapters

Summary
Automatic Translation

Here a protocol to investigate the early effects of amyloid-β (Aβ) in the brain is presented. This shows that Aβ induces clathrin-mediated endocytosis and collapse of axonal growth cones. The protocol is useful in studying early effects of Aβ on axonal growth cones and may facilitate prevention of Alzheimer's disease.

Tags

Axonal Growth Cone Collapse Early Amyloid β Effects Alzheimer's Disease Research Visualizing Analyzing Micro-scissors Mince Cerebral Cortices Embryonic Day-14 Mice Neuron Culture Medium Antibiotics Centrifuge Trypsin Incubate Neutralize DNAse Soybean Trypsin Inhibitor Solution Medium A Triturate Polished Pasteur Pipette Debris Filter Cell Count
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter