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Immunology and Infection

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JoVE Journal Immunology and Infection
Generazione di cellule Knock-out NK umano primario ed espansa utilizzando Cas9 ribonucleoproteine
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Generazione di cellule Knock-out NK umano primario ed espansa utilizzando Cas9 ribonucleoproteine

Article DOI: 10.3791/58237-v • 07:26 min • June 14th, 2018
June 14th, 2018 •
1, 2, 1, 3, 1, 1, 1, 1, 1, 1, 1, 3, 1
1Center for Childhood Cancer and Blood Disease, Nationwide Children's Hospital, 2Nuffield Division of Clinical Laboratory Sciences, Radcliffe Department of Medicine, University of Oxford, 3Center for Clinical and Translational Research, Nationwide Children's Hospital Research Institute

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Qui, presentiamo un protocollo per modificare geneticamente le cellule primarie o espanso umana naturale dell'assassino (NK) utilizzando Cas9 ribonucleoproteine (Cas9/RNP). Utilizzando questo protocollo, abbiamo generato le cellule NK umane carenti per trasformare il recettore del fattore di crescita – b 2 (TGFBR2) e ipoxantina fosforibosiltransferasi 1 (HPRT1).

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Immunologia e infezione 136 del problema primarie cellule NK umani espanso cellule NK CRISPR/Cas9 Cas9/RNP gRNAs TGFBR2 le cellule NK del TGFβ resistente
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