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Journal / Immunology and Infection / Cas9 Ribonucleoproteins kullanarak Knock-out…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Cas9 Ribonucleoproteins kullanarak Knock-out birincil ve genişletilmiş insan NK hücre üretimi
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Cas9 Ribonucleoproteins kullanarak Knock-out birincil ve genişletilmiş insan NK hücre üretimi

Article DOI: 10.3791/58237-v • 07:26 min • June 14th, 2018
June 14th, 2018 •
1, 2, 1, 3, 1, 1, 1, 1, 1, 1, 1, 3, 1
1Center for Childhood Cancer and Blood Disease, Nationwide Children's Hospital, 2Nuffield Division of Clinical Laboratory Sciences, Radcliffe Department of Medicine, University of Oxford, 3Center for Clinical and Translational Research, Nationwide Children's Hospital Research Institute

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Burada, genetik olarak Cas9 Ribonucleoproteins (Cas9/RNPs) kullanarak birincil veya genişletilmiş insan doğal öldürücü (NK) hücreleri değiştirmek için bir iletişim kuralı mevcut. Bu iletişim kuralını kullanarak, biz insan NK hücreleri dönüştürme büyüme faktörü-b reseptör 2 (TGFBR2) ve hypoxanthine phosphoribosyltransferase 1 (HPRT1) için eksik oluşturulan.

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İmmünoloji ve enfeksiyon sayı: 136 insan birincil NK hücreleri insan genişletilmiş NK hücreleri CRISPR/Cas9 Cas9/RNPs gRNAs TGFBR2 TGFβ dayanıklı NK hücreleri
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