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JoVE Journal
Biology

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下一代 16S rRNA 扩增子测序的蜱菌群鉴定
 
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下一代 16S rRNA 扩增子测序的蜱菌群鉴定

Article DOI: 10.3791/58239-v 07:21 min August 25th, 2018
August 25th, 2018

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Summary

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在这里, 我们提出了下一代测序协议 16S rRNA 测序, 使识别和鉴定微生物群落在载体内。该方法包括 DNA 的提取、扩增和条形码样品的 PCR、序列化和生物信息学, 将序列数据与系统进化信息相匹配。

Tags

生物学 问题 138 向量 微生物 16S rRNA 扩增子 下一代测序 Ixodes
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