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Kryds Microbiome karakterisering af næste generations 16S rRNA-amplikon sekventering
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Kryds Microbiome karakterisering af næste generations 16S rRNA-amplikon sekventering

Article DOI: 10.3791/58239-v • 07:21 min • August 25th, 2018
August 25th, 2018 •
1, 1
1Department of Biology, San Francisco State University

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Her præsenterer vi en next-generation sequencing protokol til 16S rRNA sekvensering, som muliggør identifikation og karakterisering af mikrobielle samfund inden for vektorer. Denne metode involverer DNA udvinding, forstærkning og stregkodesystem prøver gennem PCR, sekventering på en flow-celle, og bioinformatik at matche sekvens data til fylogenetiske oplysninger.

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Biologi spørgsmålet 138 kryds vektor microbiome 16S rRNA amplikon next-generation sequencing Ixodes
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