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Biochemistry

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JoVE Journal Biochemistry
Een High-Throughput Luciferase Assay te evalueren proteolyse van de Single-omzet Protease-PCSK9
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Een High-Throughput Luciferase Assay te evalueren proteolyse van de Single-omzet Protease-PCSK9

Article DOI: 10.3791/58265-v • 08:15 min • August 28th, 2018
August 28th, 2018 •
1, 2, 2
1Division of Cardiology, Department of Medicine, Zuckerberg San Francisco General and University of California San Francisco, 2Department of Cellular and Molecular Pharmacology and Howard Hughes Medical Institute, University of California San Francisco

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Dit protocol biedt een methode om te evalueren van de proteolytic activiteit van een intrinsiek laag-activiteit, één omzet protease in een cellulaire context. In het bijzonder is deze methode toegepast om te evalueren van de proteolytic activiteit van PCSK9, een belangrijke bestuurder van lipide metabolisme waarvan proteolytic activiteit vereist voor de ultieme hypercholesterolemic functie is.

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Biochemie kwestie 138 Proprotein convertase subtilisin/kexin type 9 protease substraat specificiteit high-throughput assay luciferase low-density lipoprotein low-density lipoprotein receptor single nucleotide polymorphism drug screening
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