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Biochemistry

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JoVE Journal Biochemistry
En høy gjennomstrømming Luciferase analysen vurdere proteolyse av Single-omsetning Protease-PCSK9
Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays
Journal (Biochemistry)
Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays
Inner Mitochondrial Membrane Sensitivity to Na+ Reveals Partially Segmented Functional CoQ Pools
Journal (Biochemistry)
Inner Mitochondrial Membrane Sensitivity to Na+ Reveals Partially Segmented Functional CoQ Pools
 
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En høy gjennomstrømming Luciferase analysen vurdere proteolyse av Single-omsetning Protease-PCSK9

Article DOI: 10.3791/58265-v • 08:15 min • August 28th, 2018
August 28th, 2018 •
1, 2, 2
1Division of Cardiology, Department of Medicine, Zuckerberg San Francisco General and University of California San Francisco, 2Department of Cellular and Molecular Pharmacology and Howard Hughes Medical Institute, University of California San Francisco

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Denne protokollen gir en metode for å vurdere proteolytiske av en egentlig lav aktivitet, enkelt omsetning protease i mobilnettet sammenheng. Denne metoden brukes spesielt for å evaluere proteolytiske av PCSK9, en nøkkelkomponent lipid stoffskifte som proteolytiske kreves for sin ultimate hypercholesterolemic funksjon.

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Biokjemi problemet 138 Proprotein konvertasene subtilisin/kexin type 9 protease substrat spesifisitet høy gjennomstrømming analysen luciferase lav tetthet lipoprotein lav tetthet lipoprotein reseptor single nukleotid polymorphism narkotika screening
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