Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / Producerer gen sletninger i Escherichia coli af…
JoVE Journal
Genetics

A subscription to JoVE is required to view this content.

JoVE Journal Genetics
Producerer gen sletninger i Escherichia coli af P1 transduktion med punktafgiftspligtige antibiotikaresistens kassetter
Chromatin Immunoprecipitation (ChIP) of Histone Modifications from Saccharomyces cerevisiae
Journal (Genetics)
Chromatin Immunoprecipitation (ChIP) of Histone Modifications from Saccharomyces cerevisiae
Mapping Mammalian 3D Genome Interactions with Micro-C-XL
Journal (Genetics)
Mapping Mammalian 3D Genome Interactions with Micro-C-XL
 
Click here for the English version

Producerer gen sletninger i Escherichia coli af P1 transduktion med punktafgiftspligtige antibiotikaresistens kassetter

Article DOI: 10.3791/58267-v • 08:13 min • September 1st, 2018
September 1st, 2018 •
1, 1, 1
1Evolution and Genetics, Department of Biosciences, University of Oslo

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Her præsenterer vi en protokol til brug af allerede eksisterende antibiotisk resistens-kassette sletning konstruktioner som grundlag for at foretage sletning mutanter i andre E. coli stammer. Sådan sletning mutationer kan mobiliseres og indsættes i den tilsvarende locus af en modtagende stamme ved hjælp af P1 bacteriophage transduktion.

Tags

Genetik sag 139 antibiotikaresistens kassette sletning mutagenese FLP recombinase P1 transduktion omplantning og forsamling modul trimeric autotransporter adhesin
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter