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Journal / Genetics / Produsere Gene slettingene i Escherichia coli av…
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JoVE Journal Genetics
Produsere Gene slettingene i Escherichia coli av P1 signaltransduksjon med Excisable antibiotikaresistens kassetter
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Produsere Gene slettingene i Escherichia coli av P1 signaltransduksjon med Excisable antibiotikaresistens kassetter

Article DOI: 10.3791/58267-v • 08:13 min • September 1st, 2018
September 1st, 2018 •
1, 1, 1
1Evolution and Genetics, Department of Biosciences, University of Oslo

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Her presenterer vi en protokoll for bruk av eksisterende antibiotikumet motstand-kassett sletting konstruksjoner som grunnlag for å gjøre sletting mutanter i andre E. coli stammer. Slike sletting mutasjoner kan være mobilisert og satt inn i tilsvarende locus mottaker belastning bruker P1 bacteriophage signaltransduksjon.

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Genetikk problemet 139 antibiotikaresistens kassett sletting mutagenese FLP recombinase P1 signaltransduksjon translokasjon og montering modul trimeric autotransporter adhesin
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