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JoVE Journal Cancer Research
A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia
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A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia

Article DOI: 10.3791/58270-v • 09:52 min • December 4th, 2018
December 4th, 2018 •
1, 1, 1, 1, 2, 1, 1, 1, 1, 1
1Dept. of Hematology, Oncology and Immunology, University of Tübingen, 2Dept. of Endocrinology, Diabetology, Clinical Pathology and Metabolism, University of Tübingen

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Summary
Automatic Translation

Chronic lymphocytic leukemia (CLL) is the most common leukemia in the western world. NFAT transcription factors are important regulators of development and activation in numerous cell types. Here, we present a protocol for the use of chromatin immunoprecipitation (ChIP) in human CLL cells to identify novel target genes of NFAT2.

Tags

Chromatin Immunoprecipitation Assay NFAT2 Target Genes Chronic Lymphocytic Leukemia Oncology Pathogenic Mechanisms Therapeutic Interventions Transcription Factors Fixation Conditions Shearing Conditions Formaldehyde Glycine PBS Stimulation Of Cells Ice-filled Box Fixation Process
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