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JoVE Journal Cancer Research
Een chromatine Immunoprecipitation Assay te identificeren van roman NFAT2 doelgenen in chronische lymfatische leukemie
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Een chromatine Immunoprecipitation Assay te identificeren van roman NFAT2 doelgenen in chronische lymfatische leukemie

Article DOI: 10.3791/58270-v • 09:52 min • December 4th, 2018
December 4th, 2018 •
1, 1, 1, 1, 2, 1, 1, 1, 1, 1
1Dept. of Hematology, Oncology and Immunology, University of Tübingen, 2Dept. of Endocrinology, Diabetology, Clinical Pathology and Metabolism, University of Tübingen

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Chronische lymfatische leukemie (CLL) is de meest voorkomende leukemie in de westerse wereld. NFAT transcriptiefactoren zijn belangrijk regulatoren van ontwikkeling en activering in vele celtypes. Hier presenteren we een protocol voor het gebruik van chromatine immunoprecipitation (ChIP) in menselijke CLL cellen te identificeren roman doelgenen van NFAT2.

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Kankeronderzoek kwestie 142 CLL NFAT transcriptiefactor doel-gen promotor chromatine immunoprecipitation
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