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JoVE Journal
Developmental Biology

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高效可扩展的人多潜能干细胞临床相容角膜缘上皮干细胞的定向分化
 
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高效可扩展的人多潜能干细胞临床相容角膜缘上皮干细胞的定向分化

Article DOI: 10.3791/58279-v 10:07 min October 24th, 2018
October 24th, 2018

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Summary

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本协议介绍了一种简单的两步法在异种和无细胞培养条件下, 从人多潜能干细胞中分化角膜缘上皮干细胞的方法。这里介绍的细胞培养方法可实现经济高效的大规模生产, 适用于角膜细胞治疗使用的临床质量细胞。

Tags

发育生物学 140 期 人类胚胎干细胞 人诱导的多潜能干细胞 角膜缘上皮干细胞 无异种 无饲养 小分子诱导 定向角膜分化
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