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Journal / Developmental Biology / Generation af menneskelige primordiale…
JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
Generation af menneskelige primordiale kønsceller-lignende celler på overfladen af Embryoid organer fra primet pluripotency induceret pluripotente stamceller
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Generation af menneskelige primordiale kønsceller-lignende celler på overfladen af Embryoid organer fra primet pluripotency induceret pluripotente stamceller

Article DOI: 10.3791/58297-v • 12:06 min • January 11th, 2019
January 11th, 2019 •
1, 1, 1, 2, 1
1Center for Cancer Research, Massachusetts General Hospital, 2Department of Molecular Genetics, Weizmann Institute of Science

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Primordiale kønsceller (PGCs) er fælles forløbere for både sæd og æg. Menneskelige embryonale PGCs er angivet fra pluripotente epiblast celler gennem interaktioner af cytokiner. Her beskriver vi en 13-dag protokol af inducerende menneskeceller transcriptomally ligner PGCs på overfladen af embryoid organer fra PRIMES-pluripotency induceret pluripotente stamceller.

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Udviklingsmæssige biologi spørgsmålet 143 primordiale kønsceller primordiale kønsceller-lignende celler (PGCLCs) inducerede pluripotente stamceller celler PRIMES pluripotency og naive pluripotency embryoid organer
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