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Journal / Immunology and Infection / Modellazione di tubercolosi in Zebrafish adulto…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Modellazione di tubercolosi in Zebrafish adulto infettato di Mycobacterium marinum
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Modellazione di tubercolosi in Zebrafish adulto infettato di Mycobacterium marinum

Article DOI: 10.3791/58299-v • 07:00 min • October 8th, 2018
October 8th, 2018 •
1, 1, 1, 1,2
1Faculty of Medicine and Life Sciences, University of Tampere, 2Oral and Maxillofacial Unit, Tampere University Hospital

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Qui, presentiamo un protocollo per tubercolosi umana modello in un adulto zebrafish utilizzando la sua naturale agente patogeno Mycobacterium marinum. Estratti di DNA e RNA da organi interni di zebrafish infetto può essere utilizzato per rivelare che il totale micobatterico carichi nel pesce e le risposte immunitarie dell'ospite con qPCR.

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Immunologia e infezione numero 140 Zebrafish tubercolosi Mycobacterium tuberculosis Mycobacterium marinum sistema immunitario infezione micobatterica qPCR
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