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Journal / Biochemistry / Anaerob Protein oprensning og kinetisk analyse…
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Biochemistry

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JoVE Journal Biochemistry
Anaerob Protein oprensning og kinetisk analyse via ilt elektrode til at studere DesB Dioxygenase aktivitet og hæmning
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Anaerob Protein oprensning og kinetisk analyse via ilt elektrode til at studere DesB Dioxygenase aktivitet og hæmning

Article DOI: 10.3791/58307-v • 08:31 min • October 3rd, 2018
October 3rd, 2018 •
1, 1, 1, 1, 1
1Department of Chemistry, Wesleyan University

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Her præsenterer vi en protokol for anaerob protein oprensning, anaerob proteinkoncentration og efterfølgende kinetic karakterisering ved hjælp af en ilt elektrode system. Metoden er illustreret ved hjælp af enzymet DesB, en dioxygenase enzym, som er mere stabilt og aktiv, når renset og gemt i en anaerobt miljø.

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Biokemi spørgsmål 140 anaerob rensning dioxygenase ilt elektrode kinetik DesB lignin ring kavalergang
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