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Biochemistry

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氧电极对 DesB 酶活性和抑制作用的厌氧蛋白纯化及动力学分析
 
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氧电极对 DesB 酶活性和抑制作用的厌氧蛋白纯化及动力学分析

Article DOI: 10.3791/58307
October 3rd, 2018

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Summary October 3rd, 2018

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在这里, 我们提出了一个协议的厌氧蛋白纯化, 厌氧蛋白浓度, 并随后的动力学表征使用氧电极系统。该方法用酶 DesB, 一种在厌氧环境中纯化和贮存时更稳定、更活跃的酶酶来说明。

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