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Journal / Developmental Biology / 精巣上体のタンパク質合成と分泌の解析…
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JoVE Journal Developmental Biology
精巣上体のタンパク質合成と分泌の解析
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精巣上体のタンパク質合成と分泌の解析

Article DOI: 10.3791/58308-v • 10:23 min • August 25th, 2018
August 25th, 2018 •
1,2, 3, 1,2, 2,4, 1,2
1Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences, University of Newcastle, 2Hunter Medical Research Institute, 3Department of Physiology, Turku Center for Disease Modeling, Institute of Biomedicine, University of Turku, 4School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle

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パラフィン埋め込まれたマウス精巣のセクションで蛋白質の検出と精巣細胞の不死化線 (mECap18) のためのプロトコルを説明するダイナミンの蛍光抗体法により局在を報告する.また精巣上体液やエアコン携帯メディアから分泌蛋白質の隔離のためのプロトコルについて述べる。

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発生生物学、問題 138、ダイナミン、精巣上体、epididymosome、エキソソーム、蛍光抗体法、タンパク質の分泌、精子、精子成熟
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