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Journal / Immunology and Infection / Isolering af ekstracellulære vesikler fra Murine…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolering af ekstracellulære vesikler fra Murine Bronchoalveolar Lavage væske ved hjælp af en ultrafiltrering centrifugering teknik
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Isolering af ekstracellulære vesikler fra Murine Bronchoalveolar Lavage væske ved hjælp af en ultrafiltrering centrifugering teknik

Article DOI: 10.3791/58310-v • 09:18 min • November 9th, 2018
November 9th, 2018 •
1, 1, 1,2, 3
1Department of Medicine, Division of Pulmonary and Critical Care, Women's Guild Lung Institute, Cedars-Sinai Medical Center, 2Department of Biomedical Sciences, Cedars-Sinai Medical Center, 3Department of Medicine, Smidt Heart Institute, Cedars-Sinai Medical Center

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Her beskriver vi to ekstracellulære vesikel isolation protokoller, ultrafiltrering centrifugering og ultracentrifugering med tæthed gradient centrifugering, at isolere ekstracellulære vesikler fra murine bronchoalveolar lavage flydende prøver. De ekstracellulære vesikler afledt af murine bronchoalveolar lavage væske ved begge metoder er kvantificeret og karakteriseret.

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Immunologi og infektion sag 141 ekstracellulære vesikler exosomes ultrafiltrering centrifugering ultracentrifugering isolation teknik bronchoalveolar lavage væske
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