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JoVE Journal Bioengineering
Gennemføre flere Imaging tilstande med et fluorescens mikroskop
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Gennemføre flere Imaging tilstande med et fluorescens mikroskop

Article DOI: 10.3791/58320-v • 08:32 min • October 28th, 2018
October 28th, 2018 •
1, 2, 2, 1,3, 4, 1,2
1Department of Biochemistry and Molecular Biology, University of Chicago, 2The Institute for Biophysical Dynamics, University of Chicago, 3Faculty of Chemistry, Wrocław University of Science and Technology, 4Nikon Instruments Inc.

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Her præsenterer vi en praktisk vejledning i opbygning af en integreret mikroskopi system, som fletter konventionelle epi-fluorescerende imaging, enkelt-molekyle påvisning funderet super-resolution imaging og multi-farve enkelt-molekyle afsløring, herunder enkelt-molekyle fluorescens resonans energioverførsel imaging, i én set-up på en omkostningseffektiv måde.

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Bioteknologi spørgsmål 140 Fluorescens mikroskopi super-resolution imaging smFRET STORM PALM JOHANNAS
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