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JoVE Journal Bioengineering
Gjennomføre flere Imaging modi med en fluorescens mikroskopet
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Gjennomføre flere Imaging modi med en fluorescens mikroskopet

Article DOI: 10.3791/58320-v • 08:32 min • October 28th, 2018
October 28th, 2018 •
1, 2, 2, 1,3, 4, 1,2
1Department of Biochemistry and Molecular Biology, University of Chicago, 2The Institute for Biophysical Dynamics, University of Chicago, 3Faculty of Chemistry, Wrocław University of Science and Technology, 4Nikon Instruments Inc.

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Her presenterer vi en praktisk guide for å bygge en integrert mikroskopi system, som sammen med konvensjonelle epi-fluorescerende bildebehandling, enkelt-molekylet gjenkjenning-basert super-oppløsning bilder og multi-farge single-molekylet gjenkjenning, inkludert Single-molekylet fluorescens resonans energioverføring bildebehandling, i et oppsett på en kostnadseffektiv måte.

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Bioteknologi problemet 140 fluorescens mikroskopi super-oppløsning bildebehandling smFRET STORM PALM TIRF
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