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Isolering av primära mus hepatocyter för begynnande proteinsyntes analys av icke-radioaktiva L-azidohomoalanine märkning metoden
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Isolering av primära mus hepatocyter för begynnande proteinsyntes analys av icke-radioaktiva L-azidohomoalanine märkning metoden

Article DOI: 10.3791/58323-v • 08:04 min • October 23rd, 2018
October 23rd, 2018 •
*1,2, *2, 2, 2, 2, 2, 2,3,4
1Department of Pharmacology and Systems Physiology, College of Medicine, University of Cincinnati, 2Division of Endocrinology, Cincinnati Children's Hospital Medical Center, 3Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, 4Department of Pediatrics, College of Medicine, University of Cincinnati
* These authors contributed equally

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Här presenterar vi ett protokoll för isolering av sunda och funktionella primära mus hepatocyter. Instruktioner för att upptäcka nedsatt begynnande proteinsyntesen av icke-radioaktiv märkning substrat tillhandahölls för att förstå mekanismerna bakom proteinsyntesen i samband med energiomsättning homeostas i levern.

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Medicin fråga 140 primära mus hepatocyter begynnande proteinsyntesen AMPK signalering rotenon L-Azidohomoalanine icke-radioaktiva metod
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