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Entrega de laser-assisted Lentivirus Gene para Mouse fertilizado ovos
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Entrega de laser-assisted Lentivirus Gene para Mouse fertilizado ovos

Article DOI: 10.3791/58327-v • 06:03 min • November 1st, 2018
November 1st, 2018 •
*1, *2, 1, 1, 1, 1, 1, 1, 3, 4, 1
1Neurobiology Laboratory, National Institute of Environmental Health Sciences, 2Comparative Medicine Branch, National Institute of Environmental Health Sciences, 3Reproductive and Developmental Biology Laboratory, National Institute of Environmental Health Sciences, 4Signal Transduction Laboratory, National Institute of Environmental Health Sciences
* These authors contributed equally

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Mouse de ovos fertilizados e embriões adiantados do palco são protegidos pela zona pelúcida, uma matriz de glicoproteína que forma uma barreira contra a entrega do gene. Este artigo descreve um protocolo para perfurar a zona com um laser para transduce células embrionárias com vetores Lentivirus e criar ratos transgénicos.

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Biologia edição 141 transgênese XYClone laser lentivirus ovos fertilizado de rato embriões do rato entrega de gene transdução
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