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Laser-assisteret Lentiviral gen levering til musen befrugtede æg
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Laser-assisteret Lentiviral gen levering til musen befrugtede æg

Article DOI: 10.3791/58327-v • 06:03 min • November 1st, 2018
November 1st, 2018 •
*1, *2, 1, 1, 1, 1, 1, 1, 3, 4, 1
1Neurobiology Laboratory, National Institute of Environmental Health Sciences, 2Comparative Medicine Branch, National Institute of Environmental Health Sciences, 3Reproductive and Developmental Biology Laboratory, National Institute of Environmental Health Sciences, 4Signal Transduction Laboratory, National Institute of Environmental Health Sciences
* These authors contributed equally

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Mus befrugtede æg og tidlige fase embryoner er beskyttet af zona pellucida, et glykoprotein matrix, der danner en barriere mod gen levering. I denne artikel beskrives en protokol for perforering af zona med en laser transduce embryonale celler med lentiviral vektorer og skabe Transgene mus.

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Biologi sag 141 transgenese XYClone laser lentivirus mus befrugtede æg mus embryoner gen levering transduktion
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