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Laser-assisted lentivirale Gene levering aan muis bevruchte eieren
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Laser-assisted lentivirale Gene levering aan muis bevruchte eieren

Article DOI: 10.3791/58327-v • 06:03 min • November 1st, 2018
November 1st, 2018 •
*1, *2, 1, 1, 1, 1, 1, 1, 3, 4, 1
1Neurobiology Laboratory, National Institute of Environmental Health Sciences, 2Comparative Medicine Branch, National Institute of Environmental Health Sciences, 3Reproductive and Developmental Biology Laboratory, National Institute of Environmental Health Sciences, 4Signal Transduction Laboratory, National Institute of Environmental Health Sciences
* These authors contributed equally

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Muis bevruchte eieren en vroege fase embryo's worden beschermd door de zona zitten, een glycoproteïne-matrix die een barrière tegen levering van het gen vormt. Dit artikel beschrijft een protocol voor de zona perforeren met een laser transduce van embryonale cellen met lentivirale vectoren en maken van transgene muizen.

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Biologie kwestie 141 Transgenese XYClone laser lentivirus muis bevruchte eieren muis embryo's gene levering signaaltransductie
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