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Journal / Biochemistry / Aislamiento de F1-ATPasa de los Protista parásito …
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JoVE Journal Biochemistry
Aislamiento de F1-ATPasa de los Protista parásito Trypanosoma brucei
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Aislamiento de F1-ATPasa de los Protista parásito Trypanosoma brucei

Article DOI: 10.3791/58334-v • 08:44 min • January 22nd, 2019
January 22nd, 2019 •
1, 1,2
1Biology Centre, Czech Academy of Science, Institute of Parasitology, 2Faculty of Science, University of South Bohemia

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Este protocolo describe la purificación de F1-ATPasa de la etapa de insectos cultivada de Trypanosoma brucei. El procedimiento produce un complejo altamente puro, homogéneo y activo adecuado para estudios estructurales y enzimáticos.

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Bioquímica número 143 F1-ATPasa Trypanosoma brucei mitocondrial ATP sintasa ATPasa de tipo F cloroformo la extracción cromatografía de líquidos
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