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JoVE Journal Biochemistry
Isolation de F1-ATPase de la protiste parasite Trypanosoma brucei
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Isolation de F1-ATPase de la protiste parasite Trypanosoma brucei

Article DOI: 10.3791/58334-v • 08:44 min • January 22nd, 2019
January 22nd, 2019 •
1, 1,2
1Biology Centre, Czech Academy of Science, Institute of Parasitology, 2Faculty of Science, University of South Bohemia

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Ce protocole décrit la purification de la F1-ATPase de la scène des insecte de Trypanosoma brucei. La procédure donne un complexe très pur, homogène et actif adapté aux études structurales et enzymatiques.

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Biochimie numéro 143 F1-ATPase Trypanosoma brucei mitochondrial ATP synthase ATPase de type F extraction au chloroforme chromatographie en phase liquide
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