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JoVE Journal Developmental Biology
Cellecyklus analyse i C. elegans kønscelleoverførsel med Thymidine Analog EdU
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Cellecyklus analyse i C. elegans kønscelleoverførsel med Thymidine Analog EdU

Article DOI: 10.3791/58339-v • 06:58 min • October 22nd, 2018
October 22nd, 2018 •
1,2, 2, 1, 2
1Department of Developmental Biology, Washington University School of Medicine, St. Louis, Missouri, 2Department of Genetics, Washington University School of Medicine, St. Louis, Missouri

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En billeddannelse-baseret metode er beskrevet som kan bruges til at identificere S-fase og analysere cellecyklus dynamics i C. elegans hermafrodit kønscelleoverførsel ved hjælp af thymidine analog EdU. Denne metode kræver ingen transgener og er kompatibel med immunfluorescent farvning.

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Udviklingsmæssige biologi spørgsmål 140 C. elegans EdU S-fase celle cyklus genom meiotiske S-fase M-fase G2-fase
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