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JoVE Journal Developmental Biology
Cell Cycle Analysis in C. Elegans Keimbahn mit Thymidin-Analog-EdU
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Cell Cycle Analysis in C. Elegans Keimbahn mit Thymidin-Analog-EdU

Article DOI: 10.3791/58339-v • 06:58 min • October 22nd, 2018
October 22nd, 2018 •
1,2, 2, 1, 2
1Department of Developmental Biology, Washington University School of Medicine, St. Louis, Missouri, 2Department of Genetics, Washington University School of Medicine, St. Louis, Missouri

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Eine Imaging-basierte Methode beschrieben, die kann verwendet werden, um S-Phase zu identifizieren und analysieren Zellzyklus Dynamik in C. Elegans Hermaphrodit Keimbahn mit dem Thymidin analoge EdU. Diese Methode erfordert keine transgene und ist kompatibel mit immunofluorescent Beflecken.

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Entwicklungsbiologie Ausgabe 140 C. Elegans EdU S-Phase Zellzyklus Keimbahn meiotische S-Phase M-Phase G2-Phase
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