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Journal / Genetics / Kweken en manipulatie van O9-1 neurale kamcellen
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Kweken en manipulatie van O9-1 neurale kamcellen
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Kweken en manipulatie van O9-1 neurale kamcellen

Article DOI: 10.3791/58346-v • 08:32 min • October 9th, 2018
October 9th, 2018 •
1, 2, 2, 1
1Molecular Physiology and Biophysics, Baylor College of Medicine, 2Department of Biochemistry and Molecular Biology, University of Southern California

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O9-1 is een cellijn van multipotente muis crest van de neurale. Hier beschrijven we gedetailleerde stapsgewijze protocollen voor kweken O9-1 cellen, O9-1 cellen in specifieke celtypes te differentiëren en genetisch manipuleren O9-1 cellen met behulp van siRNA-gemedieerde knockdown of bewerken van CRISPR-Cas9-genoom.

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Genetica CRISPR Cas9 O9-1 cellen gen knock-out gene vechtpartij neurale kamcellen
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