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La coltura e la manipolazione di cellule della cresta neurale O9-1
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La coltura e la manipolazione di cellule della cresta neurale O9-1

Article DOI: 10.3791/58346-v • 08:32 min • October 9th, 2018
October 9th, 2018 •
1, 2, 2, 1
1Molecular Physiology and Biophysics, Baylor College of Medicine, 2Department of Biochemistry and Molecular Biology, University of Southern California

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O9-1 è una linea di cellule neurali della cresta multipotenti del mouse. Qui descriviamo protocolli dettagliati passo-passo per la coltura delle cellule O9-1, O9-1 cellule differenziate in tipi cellulari specifici e manipolando geneticamente O9-1 cellule utilizzando siRNA-mediata atterramento o CRISPR-Cas9 editing genomico.

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Genetica emettere 140 CRISPR Cas9 O9-1 cellule knockout del gene le cellule della cresta neurale atterramento gene
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