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Journal / Genetics / 養殖と O9 1 神経堤細胞の操作
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養殖と O9 1 神経堤細胞の操作
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養殖と O9 1 神経堤細胞の操作

Article DOI: 10.3791/58346-v • 08:32 min • October 9th, 2018
October 9th, 2018 •
1, 2, 2, 1
1Molecular Physiology and Biophysics, Baylor College of Medicine, 2Department of Biochemistry and Molecular Biology, University of Southern California

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O9 1 は多能性マウス神経堤細胞株です。ここで O9 1 細胞を培養、特定の細胞型へ O9 1 細胞と分化および遺伝子 siRNA によるノックダウンや CRISPR Cas9 ゲノム編集による O9 1 セルを操作するための詳細なステップバイ ステップ プロトコルについて述べる。

Tags

遺伝学、問題 140、CRISPR Cas9 O9 1 細胞、遺伝子ノックアウト遺伝子ノックダウン、神経堤細胞
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