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Genetics

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Kweken en manipulatie van O9-1 neurale kamcellen
 
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Kweken en manipulatie van O9-1 neurale kamcellen

Article doi: 10.3791/58346
October 9th, 2018

Summary October 9th, 2018

Please note that all translations are automatically generated.

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O9-1 is een cellijn van multipotente muis crest van de neurale. Hier beschrijven we gedetailleerde stapsgewijze protocollen voor kweken O9-1 cellen, O9-1 cellen in specifieke celtypes te differentiëren en genetisch manipuleren O9-1 cellen met behulp van siRNA-gemedieerde knockdown of bewerken van CRISPR-Cas9-genoom.

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