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JoVE Journal
Genetics

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O9-1 神经嵴细胞的培养与调控
 
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O9-1 神经嵴细胞的培养与调控

Article DOI: 10.3791/58346-v 08:32 min October 9th, 2018
October 9th, 2018

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

O9-1 是一种有多向的小鼠神经嵴细胞系。在这里, 我们描述了 O9-1 细胞培养的详细的分步协议, 将 O9-1 细胞分化为特定的细胞类型, 利用 siRNA 介导的击倒或 CRISPR-Cas9 基因组编辑基因操作 O9-1 细胞。

Tags

遗传学 问题 140 CRISPR Cas9 O9-1 细胞 基因敲除 基因击倒 神经嵴细胞
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